Composite
Part:BBa_K415069
Designed by: Leanna Morinishi Group: iGEM10_MIT (2010-10-26)
Revision as of 03:40, 27 October 2010 by Supacalafrglstic (Talk | contribs)
pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI
This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production. This is nearly the same part as Part:BBa_K415023 except that the promoter controlling LuxI expression is Part:BBa_K415031, a less leaky version of Part:BBa_R0065.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3687
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 747
Illegal BsaI.rc site found at 2954
[edit]
Categories
Parameters
None |