Composite

Part:BBa_K415069

Designed by: Leanna Morinishi   Group: iGEM10_MIT   (2010-10-26)
Revision as of 03:39, 27 October 2010 by Supacalafrglstic (Talk | contribs)

pLux-Lac:GFP:Term:Term:PluxR/cI-OR :mCherry:Term:PtetR:LuxR:Term:Fixed-pLux/cI:LuxI

This is a LuxR/LacO-regulated GFP, LuxR/cI-regulated mCherry with LuxR/cI-regulated AHL amplification, TetR-regulated LuxR production. This is nearly the same part as Part:BBa_K415023 except that the promoter controlling LuxI expression is a less leaky version of Part:BBa_R0065.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747
    Illegal BsaI.rc site found at 2954


[edit]
Categories
Parameters
None