Part:BBa_K323135:Experience

Cells containing plasmids with Part:BBa_K323135 and Part:BBa_K323132 were transformed with Part:BBa_K323066, Part:BBa_K323153 and a commercial pBluescript plasmid without a DNA program. Cultures were incubated at 30°C for several days and samples were taken at various times. Violacein was extraced from individual samples to analyze the products and evaluate the yield of violacein production.

Figure: Bacterial cultures carrying plasmids containing a DNA program, a scrambled DNA program and no DNA program. Cultures of bacteria carrying plasmids with Part:BBa_K323132, Part:BBa_K323135 and i) plasmid containing a DNA program Part:BBa_K323066, ii) plasmid containing a scrambled DNA program Part:BBa_K323153 and iii) pBluescript plasmid without a DNA program were incubated at 30°C in a Luria Bertani media. The color of the culture containing the plasmid with the DNA program is notably more purple than the color of other two cultures.

Figure: Violacein extracts of bacterial cultures carrying plasmids containing a DNA program, a scrambled DNA program and no DNA program. Violacein was extracted from the incubated cultures. An extensive difference in color intensity is visible between the extracts of all three cultures, among which the extract of the culture containing a plasmid with the DNA program exhibits the most intensive purple color.

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