Coding
vioB-AB

Part:BBa_K349110:Design

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 03:05, 27 October 2010 by Anh (Talk | contribs) (→‎Design Notes)

vioB-AB (BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2529
    Illegal AgeI site found at 2725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 3006


Design Notes

This part has built-in BsaI cutsites surrounding the vio operon biobrick (BBa_K274002) vioB cassette. Digestion at these sites produces an A overhang at the 5' end and a B overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3

References