Part:BBa_K346087

RBS(BBa_B0034)+MerP

This part is constructed for the expression of merP, which can bind Hg(II) in the periplasm and deliver it to merT for the transmembrane transportation. Structural studies have indicated that merP functions as a monomer and binds a single Hg (II) ion via its two cysteine residues at positions 14 and 17. It then transfer the Hg(II) to the merT, which receive it by a cysteine pair in the periplasm side.

We constructed this part to increase the amount of Hg(II) that can be uptaked into the cell. Since the more Hg(II) in the cell, the more merR dimer will bind Hg(II)and be activated. Those active merR dimer will cause the gene expression under pmerT. This part can thus increase the amount of expression of genes under pmerT by increase cellular Hg(II) concentration.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 93
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 265
  
• 1000
  COMPATIBLE WITH RFC[1000]