Regulatory

Part:BBa_K337027

Designed by: Adlung L,Al Sabah J,Bayer P,Berrens R,Cristiano E,Flocke L,Kleinsorg S,Kolodziejczyk A,Macias Torre A,Mathur A,Mayilo D,Neumann S,Niopek D,Pisa R,Schad J,Schuhmacher L,Uhlig T,Wu X,Grimm D,Eils R   Group: iGEM10_Heidelberg   (2010-10-22)
Revision as of 01:33, 27 October 2010 by Dniopek (Talk | contribs)

CMV_TetO2

CMV Promoter with Tet Operator (TetO2) (BB-2, RFC12). We testet this part by dual luciferase assay in context of Part BBa_K337038 and BBa_K337046 by transfection into Hek-Trex cells, having a CMV driven TetR construct stably integrated. A complete knockdown was observed (value K3), whereas the RSV and CMV showed a stable and high expression. Transfection into other cell lines led to stable high expression, showing, that the part is working.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 605
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None