Coding
KanR-AB-Bs

Part:BBa_K349007

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)
Revision as of 01:15, 27 October 2010 by Phacking (Talk | contribs)

KanR-AB-BsaI (for construction of AB BioBytes 2.0)

BioByte Assembly Method 2.0 base plasmid v.1 containing the kanamycin resistance gene. Digestion with BsaI allows for the addition of any other ORF style part to the plasmid.


Usage and Biology

This part is used to create AB parts in the BioByte 2.0 Assembly Method.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


[edit]
Categories
Parameters
n/aKanR-AB-BsaI (for construction of AB BioBytes 2.0)