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Coding
RFP-BA-Bbs

Part:BBa_K349003

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 01:06, 27 October 2010 by Phacking (Talk | contribs)

RFP-BA-BbsI (for construction of BA BioBytes 2.0)

Base Plasmid v.2 for the construction of new BioBytes 2.0. The RFP cassette can be removed using the BbsI enzyme and a new AB ORF style part may be inserted into the plasmid allowing for creation parts for the BioBytes Assembly Method 2.0.

Usage and Biology

The Base Plasmids v.2 provide a method to create parts for BioByte Assembly Method 2.0. The RFP coding cassette provides a selection method for plasmids that have the new part inserted.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aRFP-BA-BbsI (for construction of BA BioBytes 2.0)