Coding
RFP-BA-Bsa

Part:BBa_K349002

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 01:02, 27 October 2010 by Phacking (Talk | contribs)

RFP-BA-BsaI (for construction of BA BioBytes 2.0)

Base Plasmid v.2 for the construction of new BioBytes 2.0. The RFP cassette can be removed using the BsaI enzyme and a new BA linker style part may be inserted into the plasmid allowing for testing of the part with the BioBytes Assembly Method 2.0.


Usage and Biology

This plasmid is used to create BA linker style parts for the BioBytes Assembly Method 2.0. The RFP coding cassette provides a selection method for your parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 792
    Illegal AgeI site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1086


[edit]
Categories
Parameters
n/aRFP-BA-BsaI (for construction of BA BioBytes 2.0)