Generator

Part:BBa_K325239

Designed by: Theo Sanderson and Hannah Copley   Group: iGEM10_Cambridge   (2010-10-23)
Revision as of 23:29, 26 October 2010 by Theos (Talk | contribs) (Replacing page with '{{:Templates/Cambridge-fireflycolour|colour=red|substituion=Gly326Ser}}')

Input: L-Arabinose
Output: Light (red)

pBad/araC
I0500		mutated version of
Luciferase/LRE
K325210

This part should show similarity to BBa_K325219     [[Part:BBa_K325{{{id}}}:Experience |Add Data]]       


Description
This part is based on part BBa_K325219. Two site-directed mutagenesis events have been carried out on the luciferase, an Asn286 Ser mutation was used to create part BBa_K325209 which is the green wild-type luciferase. Then a {{{substitution}}} mutation was used to create a red bioluminescence.


Colour of emission

Figure 1 - Comparison of part BBa_K325{{{id}}} against other constructs created by the E.glowli team. Note that this picture should be used as a relative comparison rather than absolute colour. All images change markedly depending on the white balance used.


Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3

[edit]
Categories
//classic/generator/uncategorized
Parameters
outputLight
positive_regulatorsL-arabinose