Part:BBa_K299810

This part is licensed under
Creative BioCommons

B0032+Invasin

Invasin (INV) plays a key role in the initiation of Yersinia enterocolytica and Yersinia pseudotuberculosis infection. Through interaction with a beta1-integrin receptor present on the surface of eucaryotic cell membranes it triggers a signal-transduction pathway leading to internalisation of the whole bacterium in the endocytosis-dependent manner. The strong affinity of listeriolysin to it’s receptor results in highly selective binding to the target molecule. Mammalian cells depleted of beta1-integrin cannot be infected.

Authors:

Cloned by Marta Błaszkiewicz under supervision of Michał Lower.

Construct design

The part consists of B0032 RBS followed by inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). The construct is a fragment of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.

Safety

Bacteria transformed with this part are invasive microorganisms. All safety precautions must be taken when manipulating with transformed strain. It involves obligatory use of laboratory gloves. Work under laminar is strongly advised. All waste should be autoclaved to avoid accidental gene transfer to other bacteria and potential rise of pathogenic organism.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 770
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1387
  
• 1000
  COMPATIBLE WITH RFC[1000]