Coding

Part:BBa_K305003:Design

Designed by: Maarten van den Nieuwenhof, Ramon Sieber, Arend Jan Suk   Group: iGEM10_Groningen   (2010-09-27)
Revision as of 22:53, 26 October 2010 by AJ (Talk | contribs) (→‎Design Notes)

Hydrophobic protein chaplin E (chpE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS sequence can be found in several B. subtilis genes and is known to efficiently initiate translation. The CDS was optimized for bacillus > GenScript software

Structural Prediction

Structural prediction of the protein without the signaling sequence by [http://zhanglab.ccmb.med.umich.edu/I-TASSER/ I-TASSER] yielded the following results. No tertiary structure has been identified by x-ray crystallography or NMR.

[http://zhanglab.ccmb.med.umich.edu/I-TASSER/output/S49452/ structural prediction] without signaling sequence

Source

Streptomyces coelicolor genomically encoded protein, codon optimized for B. subtilis and sythesized by Mr. Gene.

References

Claessen, D; Rink, R; de Jong, W et al. 2003. A novel class of secreted hydrophobic proteins is involved in aerial hyphae formation in Streptomyces coelicolor by forming amyloid-like fibrils. Genes Dev 17 1714-1726