Composite
ori+TetR

Part:BBa_K320003

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 21:48, 26 October 2010 by Spaceman (Talk | contribs)

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Origin for Asaia and resistance to Tetracycline

This BioBrick consists of two origins of replication, one for Asaia and one for E. coli, (BBa_K320000) and a resistance to Tetracycline (BBa_P1005) . It was designed to be inserted into (BBa_I51020).


Usage and Biology

This BioBrick can be used in E.Coli and also in Asaia to make the bacteria resistant to tetracycline.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


Functional Parameters

[edit]
Categories
Parameters
None