Reporter

Part:BBa_K362052

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-25)
Revision as of 17:33, 26 October 2010 by Fukuyama (Talk | contribs)

ahpC promoter - RBS - RFP - Term - Term - sufA promoter - RBS - GFP - Term - Term

This composite part contains ahpC promoter(BBa_K362001),RBS(BBa_I13507), RFP(BBa_I13507) and two terminators(BBa_I13507), sufA promoter(BBa_K362005),RBS(BBa_K121013), GFP(BBa_K121013), two terminators(BBa_K121013), ahpC promoter is controlled by OxyR protein, an oxidative stress resoponsive transcription factor.

sufA promoter has sensitivity to oxidative stress.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2911
    Illegal BamHI site found at 2900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
    Illegal AgeI site found at 1581
    Illegal AgeI site found at 1693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3603


[edit]
Categories
Parameters
None