Protein_Domain

Part:BBa_K165007:Design

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-26)
Revision as of 16:58, 26 October 2010 by TinaL (Talk | contribs)

Gli-1 DNA-binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Team Slovenia 2010 deposited a modified variant of Gli1 for isolation, purification and other experiments. We added HIS tag to N-terminal side of Gli1 sequence and N-terminal split CFP on C-terminal part of Gli1 after an extension linker. We deposited modified variant of Gli1 as Gli1_link_nCFP BBa_K323016 and additionally characterized it with several methods, including SDS-page, Western Blot and circular dichroism spectroscopy. In vitro binding of Gli1 to target DNA sequence was determined with surface plasmon resonance (SPR) and in vivo with betagalactosidase assay as a reporter.

Source

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References

Kasper M., Regl G., Frischauf A., Aberger F. 2006. GLI transcription factors: Mediators of oncogenic Hedgehog signalling. EUROPEAN JOURNAL OF CANCER 42 (2006) 437–445