Reporter
luc

Part:BBa_K389004:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 14:44, 26 October 2010 by Jaretz (Talk | contribs) (→‎References)

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Luciferase from pGL4.10[luc2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1314


Design Notes

sequence of this vector is already published by Promega [http://www.promega.com/vectors/pgl4.10.txt here]

Source

pGL4.10[luc2]

References

  • Conti E, Franks NP, Brick P (1996) [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VSR-4CJB7RR-7&_user=2459438&_coverDate=03%2F31%2F1996&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057302&_version=1&_urlVersion=0&_userid=2459438&md5=ac60aa60eaad17b2aa1c4b31f954d0a8&searchtype=a Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes], Structure, 4(3):287-98.
  • wikipedia article: http://en.wikipedia.org/wiki/Luciferase
  • Sequence of pGL4.10[luc2]: http://www.promega.com/vectors/pgl4.10.txt