Composite

Part:BBa_K404213

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 13:16, 26 October 2010 by Achim (Talk | contribs)

ViralBrick-587KO-Z34C
The Z34C antibody binding motif including a knockout of the natural HSPG tropism, ready for insertion into the 587 loop

Freiburg10 ViralBrick-logo-453-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Freiburg10 ViralBrick motif Z34C.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/miscellaneous
Parameters
None