Part:BBa_K338004

PHA Synthase Composite, Part 2/2

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains phaB1 and phaC1: BBa_K156013 & BBa_K156014. It was designed to be ligated downstream of part BBa_K338003.

Usage and Biology

Although not a finished product, it was tested in DH5α cells grown in 5mM glucose for 48 hours (5mL LB culture) at 37°C. The cells were then imaged at 100x, producing the following figures:

The small inclusion bodies suggest the production of an unknown, but possibly plastic-related, substance. A similar construct, pSYL105, caused the formation of large inclusion bodies in cells that produced plastic, lending credence to this possibility. [1]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1348
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 697
  Illegal AgeI site found at 1047
  
• 1000
  COMPATIBLE WITH RFC[1000]