Composite

Part:BBa_K338003

Designed by: Caltech iGEM 2010   Group: iGEM10_Caltech   (2010-10-26)
Revision as of 10:57, 26 October 2010 by Registry (Talk | contribs)

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PHA Synthase Composite, Part 1/2

This is half of a planned part which would contain all three PHA synthase genes required to produce [http://en.wikipedia.org/wiki/Polyhydroxybutyrate polyhydroxybutyrate] (PHB) in cells: phaA, phaB1, phaC1. This half contains only phaA: BBa_K156012. It was designed to be ligated upstream of part BBa_K338004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 338
    Illegal AgeI site found at 701
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/biosynthesis/plastic
//function/degradation
Parameters
None