Generator

Part:BBa_K346030

Designed by: Chen Yiwei   Group: iGEM10_Peking   (2010-10-14)
Revision as of 08:18, 26 October 2010 by JjunyiJiao (Talk | contribs)

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T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of lead binding peptide)

This part is composed of a T7promoter, a rbs and the periplasm display protein Dsba-MBP, which binding the lead of the enviroment. DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically. The construction of Dsba-MBP(for lead) is similar to that part for mercury binding.more details[[1]]

Here is the results of our experiments.

Pb dsab.jpg

Figure 1: The expression of DsbA+MBP. This MBP is engineered to bind lead more efficiently. We use this part to capture lead in the periplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 520
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 308
    Illegal XhoI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 520
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 520
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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