Generator

Part:BBa_K346033

Designed by: Liang Donghai Teng Xin   Group: iGEM10_Peking   (2010-10-14)
Revision as of 07:07, 26 October 2010 by Starshine (Talk | contribs)

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T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)

This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide)

Description

DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .

Dsba-MBP.jpg

Figure 1: The structure of Dsba+MBP. This protein's function is to carry MBP to the periplasm and bind heavy metal there.

Dfadsfds.jpg

Figure 2: The expression of Dsba+MBP. We can see that the 40 kd band both in the SDS-PAGE and Western blotting result.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 529
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 529
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 317
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 529
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 529
    Illegal AgeI site found at 149
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None