Reporter

Part:BBa_K346018

Designed by: Zairan Liu   Group: iGEM10_Peking   (2010-10-18)
Revision as of 05:09, 26 October 2010 by Kitties (Talk | contribs)

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PmerT promoter(88)+RBS(B0034)+lacZ alpha(BBa_I732006)

Compared to BBa_K346016, this part use lacZ alpha as a reporter instead of GFP. When treated with X-gal, the strain's color would be an indication for the concentration of Hg(II).Compared to BBa_K346017, PmerT was replaced by its mutant 88 to make strain with different sensitivity to Hg(II).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None