Part:BBa_K316008
Cleavable GFP-XylE fusion with Pveg promoter and terminator
This part contains BBa_K316007 with added double terminator BBa_B0014. This construct is designed so that the XylE activity is substantially reduced untill such a time when a TEV protease is added to the system and transcribed. TEV protease cleavable linker is positioned between the two proteins. Once the linker is cleaved, XylE is free to tetramerise and assume full activity. GFP is His tagged at the 5' end to facilitate purificaiton for in-vitro assays. Terminator BBa_B0014 has been added to comply with Biobrick standards. This particular terminator is stronger and is different from BBa_B0015.
Structure and Features
Figure I. Graphical representation of the GFP-XylE construct with associated Pveg promoter, tags, linkers and double terminator.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1229
Illegal NgoMIV site found at 1401
Illegal AgeI site found at 1752 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 785
For more information about our project please visit our wiki [http://2010.igem.org/Team:Imperial_College_London].
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