Part:BBa_K415000
pLux/cI-OR : RBS-mCherry : Term
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
BBa_K415000 represents an AND-Gate promoter controlling the expression of expressing mCherry. The promoter requires two inputs: an environment free of cI and secondly, quorum sensing, in the form of LuxR-homoserine-lactone for triggering the pLux promoter. The part was constructed via the insertion of BBa_J06702 into a BBa_R0065 backbone(pSB1A2). The following 1% agarose gel, prepared from powder and 1% TAE and stained with SYBR® Safe DNA gel stain, presents confirmation of said construction. The part was run along the standard of Hyperladder 1.
Figure 1
The following is a plasmid map of the part in the pSB1A2 backbone:
Figure 2
The fluorescence of mCherry was then quantified using FACs(Fluorescence-activated cell sorting) running two samples: -AHL and +AHL. The addition of AHL tests whether or not the circuit will respond without LuxR. The fluorescence of mCherry was measured in arbitrary units.
Figure 3
It is clear that the circuit does not respond to the addition of AHL. The following are FACs sheets:
Figure 4: -AHL
Figure 5: +AHL
Source
https://parts.igem.org/wiki/index.php?title=Part:BBa_R0065
https://parts.igem.org/wiki/index.php?title=Part:BBa_J06702
References
| None |