Device

Part:BBa_K302012:Design

Designed by: Rachel May Boyd, Phil Hall   Group: iGEM10_Newcastle   (2010-08-12)
Revision as of 02:08, 26 October 2010 by Swoodhouse (Talk | contribs) (→‎Source)

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IPTG-inducible filamentous cell formation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The stop codon was modified to a double TAA.

Source

  • yneA: Genomic DNA from Bacillus subtilis
  • Hyperspankoid from pspac, oid and hyperspank

References

Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.

Mo, A.H. & Burkholder, W.F., 2010. YneA , an SOS-Induced Inhibitor of Cell Division in Bacillus subtilis , Is Regulated Posttranslationally and Requires the Transmembrane Region for Activity ᰔ †. Society, 192(12), 3159-3173.