Part:BBa_K299503

Expression vector J23100+B0033

This part is licensed under
Creative BioCommons

This is expression vector containing B0033 under J23100 promoter. It can be used for easily expression of any protein-encoding BioBrick in RFC10 format.

Authors

Clonning:

• Ania Puławska
• Milena Bażlekowa

The lab work was supervised by:

• Michał Lower
• Ania Olchowik

Construct design

The device consists of J23100 promoter followed by B0033 RBS. Device was created by annealing of synthetic complementary oligonucleotides followed by subsequent cloning into pSB1A2 vector.

Expression strength

Expression strength of this device is based on measurements of [http://2010.igem.org/Team:Warsaw/Stage1/PromMeas promoter strength] and [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS performance] done by Team Warsaw 2010.

It's estimated to be 0,11% relative to BBa_K299509, the strongest ExpressIt! vector.

Summary for the whole ExpressIt! family is shown below:

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]