Reporter

Part:BBa_K274003:Experience

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-18)
Revision as of 23:04, 25 October 2010 by Zcbcd06 (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274003

User Reviews

E.coli turned dark green after transformation of this plasmid, it is not supposed to be so since there is no promoter upstream of the operon. We have not found the reason.

BBa_K274003 Review No.2 UCL_London, Xiang Chen

There is no XbaI restriction site in this plasmid, thus cannot be used to asseble after other parts.

Diagnostic DNA gel was run to test all 4 restriction sites. BBa_K274003 was cut with XbaI, PstI, EcoRI & SpeI, XbaI & PstI. A gel picture is shown as below.

K274003 Diagnostic Gel .jpg

UNIQfd5e993d54a11180-partinfo-00000000-QINU UNIQfd5e993d54a11180-partinfo-00000001-QINU