Template:T7RBS
Revision as of 22:44, 25 October 2010 by Mlower (Talk | contribs) (New page: {{BioCommons}} We planned to use this device to determine {{{name}}} strength relative to <partinfo>B0034</partinfo> using cell-free system. ===Authors=== Clonning: *Ania Puławska *Mile...)
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We planned to use this device to determine {{{name}}} strength relative to BBa_B0034 using cell-free system.
Authors
Clonning:
- Ania Puławska
- Milena Bażlekowa
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The device consists of BBa_I719005 T7 promoter followed by {{{name}}} community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein. Device was created by cloning BBa_I13401 behind ExpressIt! vector containing appropriate RBS.