Device

Part:BBa_K302012:Experience

Designed by: Rachel May Boyd, Phil Hall   Group: iGEM10_Newcastle   (2010-08-12)
Revision as of 22:08, 25 October 2010 by RachelBoyd (Talk | contribs) (→‎Applications of BBa_K302012)

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Applications of BBa_K302012

Graph1:
Teamnewcastle yneA168.png
Graph 1 shows that overexpression of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168.
See below: Bacillus subtilis 168 cells (left),Bacillus subtilis expressing yneA(centre) and Bacillus subtilis overexpressing yneA(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle yneA1.jpgTeamnewcastle yneA.jpg
Graph2:
Newcastle no induction.jpg
Graph 2 shows the percentage of cells at different lengths(μm)uninduced
See below: Bacillus subtilis 168 cells (left) and non-induced cells(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle noindBS.jpg
Graph3:
Newcastle 0.2 induction.jpg
Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG
See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle 0.2indBS.jpg
Graph4:
Newcastle 1IPTG.jpg
Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG
See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right)
Teamnewcastle yneA168BS.jpgTeamnewcastle 1indBS2.jpg

Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.

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