Coding

Part:BBa_K364319:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-08)
Revision as of 21:51, 25 October 2010 by Lityupix (Talk | contribs) (→‎References)

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Gal4 DBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

The construction of the DBD and pSB1C3 backbone have been done with RFC 25.

Source

Yeast transcription activator protein. Arteficially synthetized then placed into pSB1C3 carrier vector.

References

Ana Traven, Branka Jelicic & Mary Sopta. "Yeast Gal4: a transcriptional paradigm revisited". EMBO reports (2006) 7, 496 - 499.

Galen A. Collins, J. Russell Lipford, Raymond J. Deshaies & William P. Tansey. "Gal4 turnover and transcription activation". Nature 461, E7 (8 October 2009).

K. K. Rodgers and J. E. Coleman. "DNA binding and bending by the transcription factors GAL4(62*) and GAL4(149*)". Protein Sci. 1994 April; 3(4): 608–619.