Coding

Part:BBa_K364319:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-08)
Revision as of 21:46, 25 October 2010 by Lityupix (Talk | contribs) (→‎Source)

Gal4 DBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

The construction of the DBD and pSB1C3 backbone have been done with RFC 25.

Source

Yeast transcription activator protein. Arteficially synthetized then placed into pSB1C3 carrier vector.

References