Part:BBa_K323088:Experience

The 2010 iGEM team Slovenia used this part in combination with Part:BBa_K323089 containing a corresponding DNA binding protein. Both parts were inserted into the low copy vector pSB4C5 (Part:pSB4C5) by tripoint ligation to form a universal testing device for DNA binding proteins.

We have constructed 14 parts for testing of DNA binding proteins HivC (Part:BBa_K323090 and Part:BBa_K323093), Gli1 (Part:BBa_K323092 and Part:BBa_K323091), Zif268 (Part:BBa_K323094, Part:BBa_K323097 and Part:BBa_K323103), Jazz (Part:BBa_K323096 and Part:BBa_K323095), Blues (Part:BBa_K323098 and Part:BBa_K323101), PBSII (Part:BBa_K323100 and Part:BBa_K323099) and TAL (Part:BBa_K323102).

Binding of the tested proteins was determined with the beta-galactosidase assay (for a detailed description of the method see the 2010 iGEM team Slovenia wiki). The representative results gathered below have been taken from different experiments and averaged to an arithmetic mean. The β-galactosidase activity of the culture without added arabinose, which should have indicated maximal activity due to minimal expression of the DNA binding protein, was normalized to 100 percent, and the β-galactosidase activity of the culture with added arabinose was compared to the latter.

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