Part:BBa_R0084

Promoter (OmpR, positive)

Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.

Usage and Biology

In nature, this promoter is upstream of the ompF porin gene and is both activating and repressing. The regulation of ompF is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At low osmolarity, EnvZ has a low activity, producing a small concentration of OmpR-P. This OmpR-P binds to high-affinity sites on ompF, activating transcription. At high osmolarity, however, EnvZ is more active, creating a high OmpR-P concentration. OmpR-P then binds to the low-affinity sites on ompF, repressing transcription. See Notes section for further discussion of negative regulation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]