Device

Part:BBa_K323088:Design

Designed by: Tina Lebar   Group: iGEM10_Slovenia   (2010-10-20)
Revision as of 19:19, 25 October 2010 by TinaL (Talk | contribs) (→‎Source)

In vivo testing device for protein-DNA binding: part 1 (DTER_pSYN_BbsI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for the synthetic promoter pSYN containing a BbsI restriction site were designed. After annealing, the resulting fragment was ligated into a vector containing a double terminator (Part:BBa_B0015) as a back insert.

Source

The double terminator (Part:BBa_B0015) was taken from the Registry of parts and the primers for the synthetic promoter were ordered from Sigma-Aldrich.

References