Coding

Part:BBa_K322211:Design

Designed by: William Rostain and Meng Lu   Group: iGEM10_Edinburgh   (2010-09-24)
Revision as of 17:54, 25 October 2010 by WillR (Talk | contribs) (→‎Design Notes)

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Red emitting firefly luciferase mutant (356K)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the chassis at 30°C will result in considerably increased brightness.

Source

Photinus pyralis, from BBa_I712019

References

Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007).