Generator

Part:BBa_K325906:Design

Designed by: Hannah Copley and Anja Hohmann   Group: iGEM10_Cambridge   (2010-10-23)
Revision as of 15:53, 25 October 2010 by Peteremmrich (Talk | contribs)

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pbad CDEG plambda AB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4322
    Illegal AgeI site found at 4537
    Illegal AgeI site found at 5125
    Illegal AgeI site found at 6108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI site found at 6627


Design Notes

This part will produce light when induced with arabinose. It has been assembled from the parts pBAD (BBa_i0500), LuxCD (BBa_K325902), LuxEG (BBa_K325903), a RiPS generator using plambda (BBa_K081006) and LuxAB (BBa_K216008) using the method of Gibson Assembly. Therefore no BioBrick scars have been created.


Source

a

References