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Part:BBa_K322705

Designed by: Chris French and Richard Edward Partridge-Hicks   Group: iGEM10_Edinburgh   (2010-09-26)
Revision as of 15:38, 25 October 2010 by WillR (Talk | contribs)

E. coli TnaA (tryptophanase) upstream region (500bp)

This is part of a set of two biobricks (with TnaA downstream region -BBa_K322706-). It is to be used for targeting genes to the TnaA (Tryptophanase, which responsible for indole production in E. coli) with the BRIDGE recombineering method.

Any biobrick can be inserted between the upstream and downstream regions of TnaA, then inserted without leaving a marker using BRIDGE. Because it does not leave a marker, this method can be used repeatedly, and one could potentially replace the whole genome with biobricked versions.

This part is made of the 500 bases upstream of the TnaA start codon (and thus include RBS and promoter for whatever gene is inserted in its place).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/athe region upstream of the E. coli tnaA (tryptophanase) locus