Translational_Unit

Part:BBa_K322451:Design

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-09-01)
Revision as of 14:45, 25 October 2010 by WillR (Talk | contribs) (→‎References)

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Photinus pyralis luciferase mutated for increased bioluminescence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1417
    Illegal NgoMIV site found at 1458
    Illegal AgeI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Wild type sequence with three mutations which increase relative bioluminescence.

Source

DNA was synthesised to have the same amino acids as the wild type except for the three mutations.


References

Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136.