Regulatory
PompC(C)

Part:BBa_K395301

Designed by: THIPRAMPAI THAMAMONGOOD   Group: iGEM10_Tokyo_Tech   (2010-10-02)
Revision as of 13:27, 25 October 2010 by Vancouver (Talk | contribs)

PompC(C) Promoter regulated by phosphorylated OmpR

This part is regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of POmpC(WT)BBa_R0082 consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. Originally, the wild-type promoter comprises 3 distinct phosphorylated OmpR recognition sites which are binding site 1, binding site 2 and binding site 3. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 24
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None