Coding

Part:BBa_K422010

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-25)
Revision as of 08:49, 25 October 2010 by Zwickyk (Talk | contribs)

trig (AarI A-part)

Biological Background

The ribosome binding domain of the trigger factor trigA binds to the large subunit of the ribosome [1].

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. CheB-A is an A-part compatible for N-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

References

[1] Hesterkamp, Deuerling and Bukau: The Amino-terminal 118 amino acids of Escherichia coli Trigger factor constitute a domain that is necessary and sufficient for binding to ribosomes. The Journal of Biologcial Chemistry. 1997; 272:35.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 88


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Categories
Parameters
None