Composite

Part:BBa_K415151:Experience

Designed by: Shirley Galbiati   Group: iGEM10_MIT   (2010-10-24)
Revision as of 06:07, 25 October 2010 by Galbiati (Talk | contribs) (→‎Applications of BBa_K415151)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K415151

Assessing p8-GR1 incorporation into phage coat

We transformed XL1-Blue cells with BBa_K415151, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.

1023westernGR1.jpg

The purple band at 13 kD represents the p8-GR1 fusion, and is evidence of the fusion protein's incorporation into the phage coat.

User Reviews

UNIQbadecefa7a131af2-partinfo-00000000-QINU UNIQbadecefa7a131af2-partinfo-00000001-QINU