Composite

Part:BBa_K415148:Experience

Designed by: Jason Stevens   Group: iGEM10_MIT   (2010-10-24)
Revision as of 06:06, 25 October 2010 by Galbiati (Talk | contribs) (→‎Applications of BBa_K415148)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K415148

Assessing p8-Jun incorporation into phage coat

We transformed XL1-Blue cells with BBa_K415149, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.

There was no signal from the blot, indicating that inducing the part may be necessary for incorporation into the phage coat.

User Reviews

UNIQ06fee2735571614d-partinfo-00000000-QINU UNIQ06fee2735571614d-partinfo-00000001-QINU