Composite

Part:BBa_K415138:Design

Designed by: Jason Stevens   Group: iGEM10_MIT   (2010-07-22)
Revision as of 00:58, 25 October 2010 by Jstev (Talk | contribs) (→‎Design Notes)

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pLac : RBS : M13 PIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard assembly, no hiccups.

Source

We got our p3 DNA from miniprepped M13K07 DNA. We designed primers to add the biobrick prefix and suffix to p3. This p3 was then appended to the B0034 RBS to form K415108. Then we added the lac promoter to allow for expression. See the Experience page for how the part was used.

References