Composite

Part:BBa_K415151

Designed by: Shirley Galbiati   Group: iGEM10_MIT   (2010-10-24)
Revision as of 00:00, 25 October 2010 by Registry (Talk | contribs)

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K415010 + RBS : p8-GR1

This part is a composite part of BBa_K415010 (PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI) with a p8-GR1 fusion appended, where p8 is the major coat protein of M13 bacteriophage and GR1 is a leucine zipper coil that can bind to corresponding zipper coil, GR2. This part is designed to produce the fusion, which can then be displayed on the phage coat when used in a helper phage system.

The p8-GR1 part consists of an RBS, the p8 leader sequence, the zipper coil, an HA tag, a linker sequence, followed by a phage display optimized p8.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2368
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None