Coding

Part:BBa_K364310:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-09-29)
Revision as of 23:06, 24 October 2010 by Lityupix (Talk | contribs) (→‎References)

EcR LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 683
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of the LBD and Backbone have been done with RFC 25.

Source

EcR (of the nuclear hormone receptor superfamily). Arteficially synthetized then placed into pSB1C3 carrier vector.

References

1.Riddiford LM, Cherbas P, Truman JW (2000). "Ecdysone receptors and their biological actions". Vitam. Horm. 60: 1–73.

2.Henrich VC (2005). "The ecdysteroid receptor". in Sarjeet S. Gill; Gilbert, Lawrence I.; Kostas Iatrou. Comprehensive molecular insect science. Amsterdam: Elsevier. pp. 243–285.

3.Hayward DC, Bastiani MJ, Trueman JW, Truman JW, Riddiford LM, Ball EE (September 1999). "The sequence of Locusta RXR, homologous to Drosophila Ultraspiracle, and its evolutionary implications". Dev. Genes Evol. 209 (9): 564–71.

4.Koelle MR, Talbot WS, Segraves WA, Bender MT, Cherbas P, Hogness DS (October 1991). "The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily". Cell 67 (1): 59–77.

5.Devarakonda S, Harp JM, Kim Y, Ozyhar A, Rastinejad F (November 2003). "Structure of the heterodimeric ecdysone receptor DNA-binding complex". EMBO J. 22 (21): 5827–40.