Coding

Part:BBa_K364314:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-01)
Revision as of 15:56, 24 October 2010 by Mrtwister (Talk | contribs) (→‎Source)

NHR-8 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 96


Design Notes

The reconstruction of the DBD to the hinge region, LBD and Backbone will be done with RFC 25


Source

C. elegans orphan nuclear receptor. Artifically synthetized Ligand Binding Domain of C. elegans NHR-8 placed into pSB1C3 carrier vector.

References

1. Lindblom TH, Pierce GJ, Sluder AE,"A C. elegans orphan nuclear receptor contributes to xenobiotic resistance" [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VRT-438BMM3-P-1&_cdi=6243&_user=10&_pii=S0960982201002366&_coverDate=06%2F01%2F2001&_sk=%23TOC%236243%232001%23999889988%23253153%23FLA%23display%23Volume_11,_Issue_11,_Pages_R413-R450,_809-908_(1_June_2001)%23tagged%23Volume%23first%3D11%23Issue%23first%3D11%23date%23(1_June_2001)%23&view=c&_gw=y&wchp=dGLbVlW-zSkzV&md5=e1002ea78d04776b5b9ccc020f6c65e5&ie=/sdarticle.pdf Curr Biol. 2001 Jun 5;11(11):864-8.]