Generator
Part:BBa_K346089
Designed by: miao jing Group: iGEM10_Peking (2010-10-15)
T7 promoter(BBa_I719005)+MerT+MerP+MerC(the facilitation module of mercury absorption)
This part conbined MerT, MerP and MerC together under the regulation of T7 promoter. MerT,merP and merC can act together to transport Hg(II) into the cytosol to activate promoters which are dependent on intracellular Hg(II). In our bioabsorbant part, we designed this part to fulfill the function that more Hg(II) will be delivered into the cytosol so that the mercury responsive promoter(PmerT) will be activated to a great extent.It then will drive downstream gene's expression with a great expression level. In this way we have made sure the proteins that were used to bind Hg(II) are expressed in large quantity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 501
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 86
Illegal NgoMIV site found at 673
Illegal NgoMIV site found at 1094 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 79
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Categories
Parameters
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