Coding
cry11Aa

Part:BBa_K332011

Designed by: Chia-Le Meng   Group: iGEM10_NCTU_Formosa   (2010-10-24)
Revision as of 11:34, 24 October 2010 by Chialemeng (Talk | contribs)

Crystal protein gene (cry11Aa) from Bacillus thuringiensis subsp. Israelensis.

The cry weapon system produce crystal protein, targetting the wrigglers, larvae of mosquitoes. The cry11Aa gene is cloned from Bacillus thuringiensis subsp. Israelensis. The cry protein is controlled by the tetR-repressible promoter PtetR (BBa_R0040), which in turn is regulated by a temperature control system. When E.coli is released into environment (<37°C), tetR begins to degrade, resulting in the promoter PtetR (BBa_R0011) expressing downstream genes, cry11Aa (BBa_K332012) and Green Fluorescent Protein (GFP)(BBa_E0040).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/ecoli
//function/biosynthesis
//plasmidbackbone/copynumber/high
Parameters
biology
function