Composite

Part:BBa_K364330

Designed by: Endre Károly Kristóf   Group: iGEM10_Debrecen-Hungary   (2010-10-20)
Revision as of 09:02, 24 October 2010 by Mrtwister (Talk | contribs)

TRE-PolyA

Tetracycline Response Element - minimal CMV promoter - PolyA tail which is a fusion of two regulators. It is not possible to fuse coding sequences between them because of scar formation after the ligation but it can be a negative control for the expression vectors made by standard BioBrick parts from pSB1A3 (BBa_K364332, BBa_K364333).

Picture of gel electrophoresis: TRE-CMV and PolyA in pSB1C3 result a 512 bp long insert while the length of TRE-CMV insert is 321 bp.
Picture of gel electrophoresis: TRE-CMV and PolyA in pSB1A3 result a 512 bp long insert forming a plasmid which can be a negative control of the expression vector built up form standard BioBrick parts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None