Translational_Unit

Part:BBa_K497007

Designed by: Joanna Hare   Group: iGEM10_Macquarie_Australia   (2010-10-24)
Revision as of 07:17, 24 October 2010 by Registry (Talk | contribs)

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Synechocystis heme oxygenase 1 with Deinococcus radiodurans bacteriophytochrome & RBS

This is an assembly of an Deinococcus radiodurans isolated bacteriophytochrome gene with a Synechocytis heme oxygenase 1 gene. A ribosome binding site has been added. The bacteriophytochrome gene produces a protein that interacts with Biliverdin, a chromophore. The chromophore is produced by heme oxygenase degradation of heme in the heme metabolic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1108
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1108
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1108
    Illegal NgoMIV site found at 1975
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2405


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Categories
Parameters
None